ABSTRACT
OBJECTIVE@#To investigate the anti-proliferative effects of saponins prepared from Plena Clematis (PC) cultured in Fujian Province, China on 4 human tumor cell lines and its possible anti-tumor mechanism.@*METHODS@#The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line (EC9706), human hepatoma cell line (HepG-2), human oral cancer cell line (KB) and human gastric cancer cell line (BGC-823) were evaluated in vitro by thiazolyl blue (MTT) method. The inhibitory effects on EC9706 treated with different concentrations of saponins (15.62, 31.25, 62.50, 125, 250 and 500 μg/mL) were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 cells treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the cell surface by a flflow cytometer.@*RESULTS@#MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them, the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins, including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction, and the cell nuclear stained orange with EB after 250 μg/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells.@*CONCLUSION@#The saponins from PC exhibited significant cytotoxicity against human EC9706, KB, BGC-823, and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Pharmacology , Cell Cycle Checkpoints , Cell Line, Tumor , Cell Proliferation , Clematis , Chemistry , Saponins , PharmacologyABSTRACT
Objective: To investigate the effect of autologous angiogenin(ANG)-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia. Methods: Porcine models of chronic ischemia were established and were randomized into the following 3 groups: autologous marrow stromal cells transfected with Ad-ANG (Group I, n=11), autologous marrow stromal cells without transfection (Group II, n=10), and controls injected with serum-free DMEM medium (Group III, n=10). The autologous marrow stromal cells were cultured and were transfected with Ad-ANG, and the cells were labeled by CM-Dil. The cels were transplanted to the ischemia site of porcine heart. The parameters we observed included Rentrop score, ejection fraction, percentage of infracted area, CM-Dil labeled cells under fluorescence microscope, number of blood vessels and expression of ANG protein. Results: Compared with group H and IE, Group I had significantly increased Rentrop score, ejection fraction, numbers of blood vessels in the infarcted areas, CM-DiI labeled cells, and ANG protein expression, but significantly decreased infarcted area (P < 0. 05 or P < 0. 01). Conclusion: Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells, greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.
ABSTRACT
Objective To evaluate the clinical value of thoracoscopy combined with laparoscopy for the treatment of early stage esophageal carcinoma. Methods From June 2010 to December 2011, 56 patients with esophageal carcinoma receiving thoracoscopic and laparoscopic esophagectomy (TLE) in our hospital and 53 receiving conventional open esophagectomy (OE) were included in the present study. The operative procedures, postoperative complications, recurrence or metastasis rates, mortality and follow-up outcomes were compared between the two groups. Results Compared with the OE group, TLE group had significantly less blood loss, shorter hospital stay, fewer ICU cases, shorter chest tube duration, less chest drainage amount and longer operation time(P0. 05). The two groups were followed up for 3 to 21 months. TLE group had a recurrence or metastasis rate of 5. 7%, similar to that in the OE group (8. 3%, P>0. 05). The total survival rate of TLE group was 98. 1%, which was similar to that in the OE group (97. 9%, P>0. 05). Conclusion Thoracoscopic and laparoscopic esophagectomy is a safe,feasible, effective and minimally invasive method for treatment of esophageal cancer; it has less complications and quick recovery and has a similar short-term outcome to conventional open esophagectomy.